Immunohistochemistry Analysis: Representative lot data. Paraffin-embedded rat cerebellum (Fig. 1) and rat hippocampus (Fig. 2) tissues were prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:300 dilution of C7865-19A Reactivity was detected using the IHC-Select Detection Kit Staining appears to be restricted to the white matter/myelinated axons (granular layer junction) in Fig. 1and cellular processes in Fig. 2.